ThinPrep® with Imaging and Aptima HPV® MRNA - Brush/Spatula

Supply #C01 - ThinPrep w/Brush/Spatula
Test Code Test Name
58315 Pap
90934 Pap, reflex HPV, if ASCUS (ages 21 and over)
90933 Pap and HPV (cotesting for ages 30-65)
91339 Pap and HPV, CT/NG (cotesting with STI risks)
91414 Pap & HPV mRNA E6/E7, reflex HPV 16,18/45
Genotype reflex when pap is negative and HPV is detected
Out of the Vial  
11361 Chlamydia trachomatis RNA, TMA
11362 Neisseria gonorrhoeae RNA, TMA
11363 Chlamydia/N. Gonorrhoeae RNA, TMA
90521 Trichomonas vaginalis RNA, Qualitative, TMA, PAP Vial
90569 Herpes Simplex Virus, Type 1 and 2 DNA, Real-Time PCR, Pap Vial

Methodology: CYTYC Imaging System

Clinical Significance: Liquid Based Pap Testing is intended for use in the screening and detection of cervical cancer, pre-cancerous lesions, atypical cells and all other cytologic categories as defined by The Bethesda System for Reporting Results of Cervical Cytology. Pap screening is not reliable for the detection of endometrial cancer.

Additional Preferred Specimens Collection
ThinPrep Broom

Collection Guide: 

Supply: C01 - ThinPrep w/Brush/Spatula

Preferred specimen(s): Pap sample collected in Preservcyt, alcohol-based solution that serves as preservative, transport and antibacterial medium for gynecologic samples. For invitro use only.

Pap Test Lubrication Jelly Memo

Spatula Collection Instructions

1. Obtain an adequate sampling from the ectocervix using a plastic spatula. If desired, use lukewarm water to warm and lubricate the speculum. Water-soluble gel lubricant sparingly applied to the posterior blade of the speculum can be used if necessary.1 Select contoured end of plastic spatula and rotate it 360 degrees around the entire exocervix while maintaining tight contact with exocervical surface.

2. Rinse the spatula as quickly as possible into the PreservCyt solution vial by swirling the spatula vigorously in the vial 10 times. Discard the spatula.

Brush Collection Instructions

1. Obtain an adequate sampling from the endocervix using an endocervical brush device. Insert the brush into the cervix until only the bottommost fibers are exposed. Slowly rotate 1/4 or 1/2 turn in one direction. DO NOT OVER-ROTATE.

2. Rinse the brush as quickly as possible in the PreservCyt solution by rotating the device in the solution 10 times while pushing against the PreservCyt vial wall. Swirl the brush vigorously to further release material. Discard the brush.

Transport instructions for Brush or Spatula Collections

3. Tighten the cap so that the torque line on the cap passes the torque line on the vial.

4. Record the patient's name and ID number on the vial, and the patient information and medical history on the cytology requisition form.

5. Place the vial and requisition in a specimen bag for transport to the laboratory.

Transport container: Vials filled with Preservcyt

Transport temperature: Room temperature

Specimen stability for CT/NG 11363: Liquid cytology (Preservcyt® preservative (Thinprep®)

  • Room temperature: 14 days
  • Refrigerated: 30 days
  • Frozen: not established
Reject criteria:
  • Unlabeled vial
  • Leakage of fluid during transport
  • Mismatch between name of patient on vial and name on test requisition.

For additional supply or collection device information, please contact DLO's Customer Service at (800) 891-2917, option 2.

1Cervicovaginal Cytology Based on the Papanicolaou Technique; Approved Guideline – Third Edition (Clinical and Laboratory Standards Institute GP15-A3).