ANA Screen, IFA, Reflex Titer/Pattern, and Reflex to Multiplex 11 Ab CascadeMain Content
Test Code: 16814
Methodology: Immunofluorescent Assay (IFA)
Clinical Significance: Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.
Includes: The ANA Screen, IFA, Reflex Titer/Pattern, and Reflex to Multiplex 11 Ab Cascade begins with an ANA Screen, IFA.
If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge.
Additionally, five antibodies will be performed at an additional charge: dsDNA, Sm/RNP, RNP, Sm, and Chromatin.
If any of those five antibodies are positive, the cascade stops and the results are reported.
If all five of those antibodies are negative, four additional antibodies will be performed at an additional charge: SSA, SSB, Scl-70, Jo-1.
If any of those four antibodies are positive, the cascade stops and the results are reported.
If all four of those antibodies are negative, the following two additional antibodies will be performed at an additional charge: Ribosomal P and Centromere B.
Please note the cascade stops upon the first positive antibody result(s) found in a group and an interpretive message is applied based on this information. It is possible that antibodies in subsequent groups are also positive, but will not be added, billed, or reported. Please contact your local Quest Diagnostics Laboratory if you are interested in adding this additional testing.
Alternative Name(s): Mixed Connective Tissue Disease,MCTD
Supply: T01 - Red/Gray SST 8.5mL
Preferred Specimen: Serum
Preferred Volume: 1mL
Transport Container: Serum Separator Tube (SST)
Transport Temperature: Room Temperature
Specimen Stability: Room Temperature: 4 days
Rejection Criteria: Grossly icteric; Gross hemolysis; Gross lipemia
For additional supply or collection device information, please contact DLO's Customer Service at (800) 891-2917, option 2.