Test Code: 6399
CPT Code(s): 85025 *CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Methodology: Electronic Cell Sizing/Counting/Cytometry/Microscopy
Clinical Significance: CBC (includes Differential and Platelets) - A complete blood count is used as a screening test for various disease states to include: anemia, leukemia and inflammatory processes.
Alternate Name(s): Coulter CBC, Complete Blood Count
Supply: Supply #T59 EDTA (lavender-top) tube
Preferred Specimen: Whole blood from a full EDTA (lavender-top) tube *See below for alternate specimen
Note: If drawn with lead testing, EDTA (tan-top) is required.
Preferred (Minimum) Volume: Microtainer 0.5 mL / 1 mL whole blood EDTA tube
Transport Container: EDTA (lavender-top) tube
Transport Temperature: Room Temperature
Specimen Requirements: Maintain specimen at room temperature. Do not refrigerate. If multiple draw, collect EDTA (lavender-top) tube last. Traumatic draw can introduce thromboplastin and trap WBC and platelets. Refrigeration can precipitate fibrin and trap WBC and platelets.
Specimen Stability:
- Room temperature: 48 hours
- Refrigerated: 48 hours (may cause platelet clumping)
- Frozen: Unacceptable
Rejection Criteria:
- Received frozen
- Hemolysis
- What are hemolyzed specimens?
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Hemolysis occurs when the red cells are damaged during sample collection causing them to rupture. Hemolyzed serum or plasma is pale pink to red rather than the normal clear straw or pale-yellow color.
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- What causes a specimen to be hemolyzed?
- Mixing tubes too vigorously
- Placing tubes in the refrigerator without allowing 30 minutes at room temperature for complete clotting
- Exposure to heat or in a refrigerator that is too cold
- Using a needle with too small of a bore necessary for the venipuncture
- Using too large a tube when using a butterfly needle
- Not allowing sufficient time for alcohol to dry on puncture site
- Leaving the tourniquet on for longer than one minute
- How can hemolyzed specimens be prevented?
- For routine collections, use a 20–22-gauge needle
- Do not remove the needle from the vein with the vacuum tube engaged
- Do not collect a specimen in a hematoma
- Do not centrifuge the specimen for a prolonged period of time
- Draw the sample gently and evenly
- What are hemolyzed specimens?
- Clotted
Alternative Specimen(s): Whole blood from a full EDTA (tan-top) tube
For additional supply or collection device information, please contact DLO's Customer Service at (800) 891-2917, option 2.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.