
Test Code: 745
CPT Code(s): 84144
Methodology: Immunoassay
Clinical Significance: Levels increase sharply during the luteal phase of the menstrual cycle. The level increases from 9 to 32 weeks of pregnancy.
Alternative Name(s): N/A
Supply: #T157 Red/Yellow SST 8mL
Preferred Specimen: Serum
Preferred Volume: 2mL
Transport Container: Serum Separator Tube (SST)
Transport Temperature: Room Temperature
Specimen Stability: Room Temperature: 72 hours
SPECIAL INSTRUCTIONS: DHEA supplementation causes a false elevation of values in this standard immunoassay method for progesterone. Physicians using progesterone levels as a criterion for triggering ovulation in an IVF cycle in patients supplemented with DHEA should assess progesterone levels using a LC/MS/MS Progesterone assay, such as test code 17183 performed at Quest Diagnostics San Juan Capistrano.
Rejection Criteria:
- Hemolysis
- What are hemolyzed specimens?
- Hemolysis occurs when the red cells are damaged during sample collection causing them to rupture. Hemolyzed serum or plasma is pale pink to red rather than the normal clear straw or pale-yellow color.
- What causes a specimen to be hemolyzed?
- Mixing tubes too vigorously
- Placing tubes in the refrigerator without allowing 30 minutes at room temperature for complete clotting
- Exposure to heat or in a refrigerator that is too cold
- Using a needle with too small of a bore necessary for the venipuncture
- Using too large a tube when using a butterfly needle
- Not allowing sufficient time for alcohol to dry on puncture site
- Leaving the tourniquet on for longer than one minute
- How can hemolyzed specimens be prevented?
- For routine collections, use a 20–22-gauge needle
- Do not remove the needle from the vein with the vacuum tube engaged
- Do not collect a specimen in a hematoma
- Do not centrifuge the specimen for a prolonged period of time
- Draw the sample gently and evenly
- What are hemolyzed specimens?
- Serum stored on barrier gel for more than 72 hours. Do not order progesterone testing as an add on to a barrier gel specimen older than 72 hours. Progesterone binds to barrier gel causing decreased values.
- Specimens exceeding stability
- Specimens other than serum
- Unlabeled or improperly labeled specimens
For additional supply or collection device information, please contact DLO's Customer Service at (800) 891-2917, option 2.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the Payor being billed.