Mycobacteria, Culture, with Fluorochrome Smear

Mycobacteria, Culture, with Fluorochrome Smear

Supply #U02 90mL Sterile w/ Green Lid
Supply # S12 E-swab, Routine, white cap
Supply #F57 Stool Specimen Container with Red Lid

Test code: 4554

CPT code(s): 87116, 87206

Clinical significance:
There are more than 70 species of mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic, granulomatous disease caused by M. tuberculosis. Identification, and, if appropriate, antibiotic sensitivity, is required to initiate necessary therapy.

Includes:

Mycobacteria Culture, Acid-Fast Bacilli Stain

An additional charge for Concentration (CPT code(s): 87015) will be added as appropriate.

Identification will be performed at an additional charge for each organism
CPT code(s) 87118: Matrix Assisted Laser Desorption/Ionization-Time of Flight orCPT code(s) 87153: AFB Sequencing

If TB is isolated for the first time or after three months after the first isolate from a patient, susceptibilities will be performed at an additional charge (CPT code(s): 87190 per drug.

Methodology: Continuous Monitoring Broth Culture System and Conventional Culture • Microscopic Exam with Fluorochrome Stain

Alternative name(s): Acid Fast Bacilli, AFB (Acid Fast Bacilli), Tuberculosis, TB


 

Collection Guide

Supply: 

  • #U02 90mL Sterile w/ Green Lid
  • #S12 E-swab, Routine, white cap *Only if Specimen type is suboptimal
  • #F57 Stool Specimen Container with Red Lid

Preferred specimen(s): 10 mL body fluids or lower respiratory tract specimens or
10 mL gastric lavage or
2 mL CSF or
2 grams fresh (unfixed) tissue or
40 mL urine collected in a sterile leak-proof container or
1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile leak-proof container or
1 gram or 1 mL stool collected in a sterile leak-proof container

See the alphabetical listing in the Quest Microbiology section of Specimen Collection & Transport Guide for further instructions.

Minimum Volume: 2 mL body fluids or lower respiratory tract specimens • 2 mL gastric lavage • 1 mL CSF • 1 gram fresh (unfixed) tissue • 20 mL urine collected in a sterile leak-proof container • 1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile leak-proof container • 1 gram or 1 mL stool

Transport temperature: Refrigerated (cold packs)

Specimen stability:

  • Room temperature: Unacceptable
  • Refrigerated: 5 days
  • Frozen: Unacceptable

Reject criteria: 

  • 24 hour pooled urine or sputum
  • Dry swabs
  • Specimens received in alcohol, formalin, EDTA, lithium heparin or conventional blood culture bottles
  • Throat/oral/sputum swabs from non-cystic-fibrosis patients
  • Urine specimens in preservative
  • Specimens received in expired transport
  • Specimens that leaked during transit
  • Specimens broken in transit

For additional supply or collection device information, please contact DLO's Customer Service at (800) 891-2917, option 2.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.

Additional names
Acid Fast Bacilli, AFB (Acid Fast Bacilli)
Tuberculosis, TB
CPT Code(s)
87116
87206
Test Code
4554